The scene hasn't changed at all during the process, 100 frames, the particle counts were exactly the same (14k) and I cleaned the data folder before each test too just in case Rf takes a bit longer to overwrite files (not sure if it does or not).
Test 1
With GUI updates on the sim took 4 mins.
With GUI updates off (ALT-D) it took 8 mins.
With command line it took 34 secs.
Test 2
I did another test because maybe I'd gotten the results mixed up.
With GUI updates on the sim took 10:52 mins.
With GUI updates off (ALT-D) it took 14 mins.
With command line it took 42 secs.
Test 3
I restarted Rf:
With GUI updates on the sim took 1:47 mins.
With GUI updates off (ALT-D) it took 5:24 mins.
With command line it took 41 secs.
Test 4
I re-ran the test (without restarting Rf):
With GUI updates on the sim took 8:30 mins.
With GUI updates off (ALT-D) it took 12:16 mins.
With command line it took 40 secs.
In every test having openGL off took longer, which is strange in itself but to get large inconsistent sim times is very strange.
Of course, the CL reigned supreme in each test
